Safety assessment of soybean bioactive peptides

The aim of the study was to investigate the acute and genetic toxicity of soybean bioactive peptides. The acute toxic test, Ames test, micronucleus test, comet assay, spermatogonial chromosomal aberrations test were used to evaluate the acute toxicity classifi cation and genetic toxicity of soybean bioactive peptides. Results showed that the oral maximum tolerance dose of mice(MTD) was 20 g/kg, and the tested soybean bioactive peptides belong to non-toxic. The results of Ames test were negative in Salmonella typhimurium at any of the concentrations tested with or without an S9 activation system(P0.05). Soybean bioactive peptides did not exhibit any cell toxicity in mouse bone marrow cells in a micronucleus assay with negative results of micronucleus test(P0.05). There were not significant difference in the chromosomal abnormality rate and spermatogonial chromosomal aberrations test between the soybean bioactive peptides and the negative control.