Establishment of fast PCR assay 5 species genes for Streptococcus Suis serotype 2 and its application to detect strains isolated from Jiangsu

Objective To develop the fast PCR for detecting 5 species genes(16s rRNA,cps,mrp,ef and sly) of Streptococcus suis serotype 2.Methods According to the five pairs of specific primers,established the method by using efficient polymerase and optimize reaction program.Results It spent 30 minutes in detecting five species gene of Streptococcus suis serotype 2 in one PCR system.It is quick and sensitive.The specificity and fidelity were the same as General PCR,but it spent 1/3 time.Conclusion This method can be applied to epidemiological surveillance and rapid detection of outbreaks for Streptococcus suis serotype 2.