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2011•Experimental and Laboratory MedicineRequires access

Analysis of the genotypes among different strains of common Mycobacteria based on 16S rRNA gene and 16S-23S rRNA gene internal transcribed spacer sequences

Yan Lixia

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Abstract

Objective To analyze the genotypes of different strains of common Mycobacteria.Methods The different genes from 97 strains introduced Mycobacteria composed of 7 species DSMZ/ ATCC were detected using 16S rRNA gene and 16S-23S rRNA internal transcription space(ITS) sequence method.Results 13 M.phlei strains,18 M.fortuitum strains,17 M.smegmatis strains,8 M.gordonae strains,9 M.chclonae strains,15 M.kansasii strains and 17 M.farcinogenes strains could be discriminated to 3,6,4,3,3,2 and 1 genotypes by16S rRNA gene;and discriminated to 3,15,7,3,4,3 and 5 genotypes by 16S-23S rRNA ITS gene respectively.Conclusions The analysis of 16S rRNA gene and 16S-23S rRNA ITS gene sequence is reliable method to discriminate Mycobacteria.The polymorphism of 16S-23S rRNA ITS gene is higher than that of 16S rRNA gene in intraspecies of Mycobacteria.

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Objective To analyze the genotypes of different strains of common Mycobacteria.Methods The different genes from 97 strains introduced Mycobacteria composed of 7 species DSMZ/ ATCC were detected using 16S rRNA gene and 16S-23S rRNA internal transcription space(ITS) sequence method.Results 13 M.phlei strains,18 M.fortuitum strains,17 M.smegmatis strains,8 M.gordonae strains,9 M.chclonae strains,15 M.kansasii strains and 17 M.farcinogenes strains could be discriminated to 3,6,4,3,3,2 and 1 genotypes by16S rRNA gene;and discriminated to 3,15,7,3,4,3 and 5 genotypes by 16S-23S rRNA ITS gene respectively.Conclusions The analysis of 16S rRNA gene and 16S-23S rRNA ITS gene sequence is reliable method to discriminate Mycobacteria.The polymorphism of 16S-23S rRNA ITS gene is higher than that of 16S rRNA gene in intraspecies of Mycobacteria.

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Available abstract

Objective To analyze the genotypes of different strains of common Mycobacteria.Methods The different genes from 97 strains introduced Mycobacteria composed of 7 species DSMZ/ ATCC were detected using 16S rRNA gene and 16S-23S rRNA internal transcription space(ITS) sequence method.Results 13 M.phlei strains,18 M.fortuitum strains,17 M.smegmatis strains,8 M.gordonae strains,9 M.chclonae strains,15 M.kansasii strains and 17 M.farcinogenes strains could be discriminated to 3,6,4,3,3,2 and 1 genotypes by16S rRNA gene;and discriminated to 3,15,7,3,4,3 and 5 genotypes by 16S-23S rRNA ITS gene respectively.Conclusions The analysis of 16S rRNA gene and 16S-23S rRNA ITS gene sequence is reliable method to discriminate Mycobacteria.The polymorphism of 16S-23S rRNA ITS gene is higher than that of 16S rRNA gene in intraspecies of Mycobacteria.

Key concepts: 23S ribosomal RNA, 16S ribosomal RNA, Biology, Internal transcribed spacer, Gene, Ribosomal RNA, Genotype, Microbiology

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Analysis of the genotypes among different strains of common Mycobacteria based on 16S rRNA gene and 16S-23S rRNA gene internal transcribed spacer sequences — Research Paper | ScholarLens