Analysis of the genotype of 54 reference strains of Mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences

Objective To analyze genotype of 54 reference strains of mycobacteria by 16S-23S rRNA Internal Transcription Space(ITS) sequence analysis.Methods The genotype of 54 reference strains of mycobacteria were analyzed by 16S-23S rRNA ITS sequence analysis.Then the phylogenetic tree was constructed and calculated similarly.Results The sequencing of 16S-23S rRNA ITS of the 54 reference strains of mycobacteria was finished.All the mycobacteria could be discriminated except that M.flavescens and M.pulveris,M.agri and M.chitae,M.thermoresistibile and M.parafortuitum,M.obuense and M.vaccae,M.duvalii and M.porcinum,M.aurum and M.sphagni,M.ulcerans and M.marinum,M.komossense,2 strains of M.tuberculosis and M.microti,M.africanum.Conclusion 16S-23S rRNA ITS sequence analysis is a reliable method to discriminate mycobacteria.The study of the genotype of the 54 reference strains of Mycobacteria is a compensation for the gene database.