Determination of trace phenol in environmental water samples by enzymatic catalytical spectrofluorimetry

A high sensitive and simple spectrofluorimetric method for the determination of phenol based on its inhibitory effect on the hemoglobin-catalyzing reaction of H2O2 and L-tyrosine was developed.The concentration of phenol is in linear relationship with the fluorescence quenching(ΔF) of system under the optimal experimental conditions.The calibration graph is linear in the range from 3.0 to 70 μmol /L with the detection limit of 1.64 × 10- 6μmol/L.The relative standard deviation for 11 replicate determination of 15 μmol/L phenol was 3.0%.This method can be used for the determination of phenol in environmental water samples.