Co-Expression of Hepatitis B Virus Proteins and Green Fluorescent Protein After Eukaryotic Cell in vitro and Hydrodynamics-Based in vivo Transfection

Objective:To construct pCX-EGFP-HBVadr2.0 eukaryotic expression vector harboring 2 copies of hepatitis B virus(HBV) genomic DNA and enhanced green fluorescent protein(EGFP) gene and investgate the co-expression in vitro and in vivo.Methods:The eukaryotic expression vector pCX-EGFP-HBVadr2.0 was constructed based on plasmids of pBR322-HBVadr2.0 harboring 2 copies of HBV genomic DNA and vector pCX-EGFP containing CMV-IE enhancer,chicken β-action promoter and EGFP by recombination DNA technique.The HBV genomic DNA and EGFP gene expressing in transfected L02 cell line in vitro and hydrodynamic injected mouse in vivo were studied.Results:The eukaryotic expression vector pCX-EGFP-HBVadr2.0 was constructed,and could expressed in vitro and in vivo.Conclusion:The HBV eukaryotic expression vector carrying EGFP reporter gene to generate transgenic mice was constructed.It should be practicable and effective in breeding and estimation by using HBV transgenic mice with the EGFP.