Construction of a Plasmid of HBV Core Antigen-EGFP Fusion Protein and Its Expression in Hep G_2 Cell

Objective To study the transient expression of HBV core antigen and enhanced green fluorescent protein (EGFP) in Hep G 2 cells transfected by a constructed plasmid (pHBc-EGFP). Methods The core antigen gene of HBV was cloned into eukaryotic expression vector pEGFP-N 1, with which Hep G 2 cells were transfected by liposome transfection method. The expression of fusion gene was analyzed by fluorescent microscopy, RT-PCR and immunohistochemistry method, respectively. Results Recombinant plasmid pHBc-EGFP expressed the HBV core antigen-EGFP fusion protein in Hep G 2 cells transiently. Conclusion pHBc-EGFP is constructed successfully, and the expression of EGFP can serve as an indicator reflecting the expression of HBV core antigen gene.