Influences of Tongluojiunao Oral Solution on learning and memory ability and synaptophysin expression in AD rats

Objective To investigate the possible mechanism of anti-Alzheimer's disease(AD) through studying the influences of Tongluojiunao Oral Solution(TOS) on the learning and memory ability and synaptophysin(SYP) expression in rat AD model.Methods Male SD rats were randomly divided into the blank group,sham-operation group,model group,donepezil hydrochloride group(DH group) and high-dose,mid-dose and low-dose TOS groups(12,24,48 mg/kg) .The rat model of AD was established by hippocampus stereotaxis injection of condensedβ-amyloid protein(β-AP 1-40 ) .After 4 weeks the period of find platform and times of passing through original platform were detected in rats by applying Morris water maze.The expression of SYP in hippocampus was detected by using Western blotting method,and the changes of hippocampus ultrastructure were observed with electron microscope.Results In the model group,compared with the sham-operation group,the average escape latency was longer significantly and times of crossing over original platform reduced significantly(all P0.01) .In all TOS groups,compared with the model group,the average escape latency was shorter significantly and times of passing through original platform increased significantly(all P0.01) .In the model group,comparedwith the sham-operation group,the SYP expression in hippocampus decreased significantly(P0.05) . In all TOS groups,compared with the model group,the SYP expression in hippocampus increased significantly(P0.05) .The outcomes of electron microscope showed neuronal degeneration in hippocampus in the model group,while TOS relieved the pathological changes of hippocampus neurons in rats.Conclusion TOS can improve the learning and memory ability in AD rats,which may be related to that it can up-regulate SYP expression.