Simple DNA Sequencing of Basidiocarps and Their Mycelia from Gill Fungi

Objective:DNA homogeneity in the edible fungi was studied to identify genetic relationship between basidiocarps and their mycelia from gill fungi.Method:The DNA fingerprintings were compared mutually among different tissues of basidiocarps and their mycelial isolates of Lentinula edodes and Pleurotus ostreatus mushrooms in the studies.Result:The results showed that there were same DNA fingerprinting patterns among pileus(including lamellae),lamellae,and stipe from one Lentinula edodes or Pleurotus ostreatus basidiocarp,and between the mycelial isolates and their original carpophores.However,there were a little DNA variation among basidiocarps of different source,and their similarity coefficients varied from 0.886 to 0.986 for Lentinula edodes mushrooms tested,and from 0.779 to 0.976 for Pleurotus ostreatus mushrooms tested with different colors and source.Conclusion:The RAPD(Random Amplified Polymorphic DNA)-PCR analyses using 12 arbitrary decamer nucleotide primers demonstrated that there exists DNA homogeneity in the edible fungi Lentinula edodes and Pleurotus ostreatus mushrooms,which provides the genetic basis for mycelial isolates identification by DNA fingerprinting comparison between isolates and their original carpophores.The results also indicated that Lentinula edodes mushrooms tested and their mycelial spawn kept the constant DNA fingerprinting patterns in the ins and outs of cultivation.