Construction and identification of recombinant plasmid pQE31-HPV16 L1

Objective To construct a recombinant plasmid pQE31 HPV16 L1 for obtaining the HPV16 L1 protein expressed in E.coli and developing the genetically engineered vaccine.Methods PCR was used to amplify the HPV16 L1 gene from plasmid pCR2.1 HPV16 L1 in which the HPV16 L1 was cloned. A new plasmid, pQE31 HPV16 L1, was then constructed by inserting the amplified HPV16 L1 gene into pQE31, a prokaryotic expression vector. Restriction analysis and sequencing were used to confirm the structure of pQE31 HPV16 L1.Results A DNA fragment in size of 1.5Kb was amplified. Restriction analysis showed that the amplified gene was inserted into pQE31 correctly. Sequencing showed there was no mutation in both ends of the inserted L1 gene.Conclusion The plasmid pQE31 HPV16 L1 is constructed successfully.