CONSTRUCTION AND IDENTIFICATION OF RECOMBINANT PLASMID PQE30-HPV58L1

Objective:To construct a recombinant prokaryotic expression vector pQ30 HPV58L1 for obtaining the HPV58 L1 protein expressed in E coli and developing the genetic engineering vaccine.Methods:The full length L1 coden region of HPV58 was amplified by PCR,and cloned into pUC19,sequenced.The recombinant expression vector pQE30 HPV58L1 was then constructed by inserting the HPV58 L1 gene into pQE30.Restriction analysis was used to confirm the exact ligation of pQE30 HPV58L1 Results:A 1 600 bp DNA fragment was amplified with PCR.Recombinant plasmid pUC19 HPV58L1 and pQE30 HPV58L1 were constructed and sequence analysis revealed the same homology to published data in GenBank.Restriction analyses of pQE30 HPV58L1 were consistent with the theoretically predicted results.Conclusion:We successfully constructed the recombinant prokaryotic expression vector pQE30 HPV58L1