Expression of Human Mature Prion-like Protein Doppel in Escherichia coli

HomDpl,the encoding gene of human mature prion-like protein Dopple,was amplified by PCR with the plasmid HoPrnd-T including the ORF of the human Prnd gene as a template.Then HomDpl was inserted into the EcoRⅠ-XhoⅠsite of pGEX-6P-1 vector to generate recombinant expression plasmid pGEX-homDpl.The pGEX-homDpl plasmid was transformed into host strain E.coli BL21(DE3) through electroporation.1.0 mmol/L IPTG was used to induce the recombinant E.coli to express homDpl protein.SDS-PAGE analysis demonstrated that homDpl protein was overexpressed in E.coli.