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[Expression of rat amelogenin gene in Escherichia coli].

Sui W, X Zhang, Liu X

Abstract

OBJECTIVE: To detect the expression of amelogenin (Am) gene in Escherichia coli (E. coli). METHODS: The cDNA fragmain of Am gene was obtained with EcoRI and Xhol I from the plasmid PUC18/Am. The fragment was inserted into prokaryotic gene fusion vector PGEX-4T-2 and an expression plasmid PGEX-4T-2/Am was constructed. PGEX-4T-2/Am was induced by IPTG for 4 h. RESULTS: 15% SDS-PAGE revealed a new forein protein band near 27,000. CONCLUSION: The constructed plasmid expresses Am gene in E. coli.

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Key concepts: Amelogenin, Plasmid, EcoRI, Escherichia coli, Molecular biology, lac operon, Biology, Complementary DNA