Expression of Matrix Metalloproteinase 9/13(MMP-9/13)and Tissue Inhibitor of Metalloproteinase 1/2(TIMP-1/2)in Liver Fibrosis Induced by Porcine Serum and Xiayuxue Decoction on It

Objective:Changes of protein expression of MMP-9/13 and TIMP1/2 in fibrotic liver tissue induced by porcine serum and effect of Xiayuxue Decoction on it are investigated in this study to explore mechanism of Xiayuxue Decotion in reversing it.Methods:Liver fibrosis in rats was induced by intraperitoneal injection of porcine serum.After 8wk of modeling,rats were given Xiayuxue Decoction for 4 weeks.Rats were sacrificed and sample was obtained for determination at 1 wk,2 wk,4 wk,8 wk and the end of 12 wk of modeling for determination.Results:High activity of MMP-2/9 and high expression TIMP-1 characterizes this liver fibrosis model from beginning to the end.Decreased protein expression of MMP-9/13 in early stage and increased protein expression of MMP-9/13 in early stage and increased protein expression of MMP-9/13 affect by protein expression TIMP-1/2.Xiayuxue Decoction can increase activity of MMP-9,promote expression of MMP-9/13,inhibit expression of TIMP-1/2.Conclusions:Imbalanced MMP-9/13 and TIMP-1/2 expression is seen in this model.The anti-liver fibrosis mechanism of Xiayuxue Decoction is regulating MMP-9/13 and TIMP-1/2 expression to degrade excessive deposition of extracellular matrix.