Effect of stasis in collaterals of renal Chinese medicine compound prescription on the renal expressions of MMP-9,TIMP-1 and TGF-β_1mRNA in glomerulosclerosis rats

Objective To investigate the effect of the Chinese medicine compound prescription on the MMP-9,TIMP-1 and TGF-β1mRNA in glomerulosclerosis(GS)rats.Methods GS rat model was established by unilateral nephrectomy and adriamycin injection,in which 24h urinary protein of rats were observed,the expressions of matrix metalloproteinase-1(MMP-1),tissue inhibitor of metalloproteinase 1(TIMP-1)were detected by ElISA and the ratio of MMP-9/TIMP-1 was calculated,and the expression of transforming growth factor-β1mRNA(TGF-β1 mRNA)was detected by fluorescence quantitative PCR for each group of rats.Results The 24 hour urinary protein of all rats in the Chinese medicine groups was lower than the model group(P0.05),the expression of TIMP-1(P0.05)was inhibited,MMP-9(P0.05)was increased,the ratio of MMP-9/TIMP-1(P0.05)was enhanced and the expression of TGF-β1 mRNA(P0.05)was inhibited.Conclusion The Chinese medicine compound prescription,which is able to remove blood stasis and reducing phlegm and disperse stagnated mass,is protective to GS rats,and whose mechanism might be related to increasing the degradation of ECM,decreasing the expression of TGF-β1 mRNA.