Influence of transplanting microencapsulated rabbit sciatic nerve tissue on spinal cord injured neurons in adult rats

Objective:To observe the influence of the NOS expression on the spinal cord semi-transection injury in adult rats after transplantation of the microencapsulated rabbit sciatic nerve tissue.Methods:A total of 90 adult Sprague-Dawley(SD) rats were randomly divided into 3 groups and were studied with Nissl staining and NADPH- d histochemistry.The staining results were analyzed with the image analysis system.Results:After spinal cord injury,the number of the neurons was reduced and the Nissl substance was lost or dissolved.On the 14th day, the number of the Nissl stained neurons increased remarkably and their volume was enhanced.These changes be- came most evident in group implanted with microencapsulated nerves tissue.NOS positive reaction in microencap- sulated nerves tissue implanted group and nerves tissue implanted group was superior to that in non-implanted group on the 3rd day after injury.On the 14th day,cell number with NOS positive reaction in nerves tissue im- planted group increased rapidly,while that in microencapsulated nerves tissue implanted group showed a steady increasing tendency.Conclusion:Xenotransplantation of microeneapsulated sciatic nerve tissue/cells can promote NOS expression in the earlier period,inhibit NO over dose creations anaphase,and improve the functional recov- ery,which is helpful for the repair and the regeneration of the injured spinal cord.