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2010•Zhongguo zhongyiyao xinxi zazhiRequires access

Study on quality standard for Shuanghuangqing Powder.

Guangbin Liu, Zhang Xun, Liu XiaoShuan

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Abstract

Objective To establish the quality standard of Shuanghuangqing Powder.Methods Artificial Calculus Bovis,Cortex Phellodendri Chinensis,Indigo Naturalis,Radix Glycyrrhizae,Radix Gentianae in Shuanghuangqing Powder were identified by TLC,and content of berberine hydrochloride was determined by HPLC.Results The method for identification had good specificity and repeatability.There was no interference in blank control.The berberine hydrochloride determination method had good linearity from 0.080 2 to 0.802 μg(r=0.999 7).The average recovery was 98.52% and the relative standard deviation(RSD) was 1.05%(n =9).Conclusion The qualitative and quantitative method is simple,specific,accurate and reliable.It can be used to effectively control the quality of Shuanghuangqing Powder.

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Objective To establish the quality standard of Shuanghuangqing Powder.Methods Artificial Calculus Bovis,Cortex Phellodendri Chinensis,Indigo Naturalis,Radix Glycyrrhizae,Radix Gentianae in Shuanghuangqing Powder were identified by TLC,and content of berberine hydrochloride was determined by HPLC.Results The method for identification had good specificity and repeatability.There was no interference in blank control.The berberine hydrochloride determination method had good linearity from 0.080 2 to 0.802 μg(r=0.999 7).The average recovery was 98.52% and the relative standard deviation(RSD) was 1.05%(n =9).Conclusion The qualitative and quantitative method is simple,specific,accurate and reliable.It can be used to effectively control the quality of Shuanghuangqing Powder.

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Available abstract

Objective To establish the quality standard of Shuanghuangqing Powder.Methods Artificial Calculus Bovis,Cortex Phellodendri Chinensis,Indigo Naturalis,Radix Glycyrrhizae,Radix Gentianae in Shuanghuangqing Powder were identified by TLC,and content of berberine hydrochloride was determined by HPLC.Results The method for identification had good specificity and repeatability.There was no interference in blank control.The berberine hydrochloride determination method had good linearity from 0.080 2 to 0.802 μg(r=0.999 7).The average recovery was 98.52% and the relative standard deviation(RSD) was 1.05%(n =9).Conclusion The qualitative and quantitative method is simple,specific,accurate and reliable.It can be used to effectively control the quality of Shuanghuangqing Powder.

Key concepts: Berberine hydrochloride, Quality standard, Radix (gastropod), Repeatability, Chromatography, Relative standard deviation, High-performance liquid chromatography, Chemistry

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