Research in extraction methods for γ-amino butyric acid in plant products

Objective:To establish the pretreatment method for extraction in γ-aminobutyric acid of plant products.Methods:Screening of the best extraction method,and we made further research on some facts in the extraction process(the concentration of sulfosalicylic acid,shock time in water bath,temperature,extraction time,ect.).Identified the extraction method that are with the concentration of 5% sulfosalicylic acid,in hot water bath,standing 12 h,and finally in automatic amino acid analyzer to analyze quantitative.Results:The sulfosalicylic acid method,including the recovery rate was up to 98.48% in internal standards,compared with hydrochloric acid hydrolysis increased 13.54%～13.77%,compared with ethanol extraction by 7.42%～8.06%.Optimization of sample extraction,the average recovery was 94.4%～99.81%.The relative standard deviation(RSD) was 0.23%.Conclusion:The method of high recovery and good reproductbility,is simple and reliable preprocessing program.