Screening and Identification of Antagonistic Microorganisms Against Pyricularia grisea

The leaves or seeds of rice were immerged in NA cultural solution and ground. The supernatant was lined on NB agar and cultured for 12 h, 356 single-clone isolates were obtained from the cultures. After these isolates were cultured in NB cultural solution for 3d, their supernatant was used for test of antagonism against Pyricularia grisea. Two significantly antagonistic isolates were gained (their inhibitive rate against germination of conidia of P.grisea being 82.5% and 100%, respectively), and identified as Bacillus subtilis and B.pumillus according to Bergey's manual of systematic bacteriology.