2011•Acta Agrestia SinicaRequires access

Genetic Diversity of Beauveria bassiana on Loxostege sticticalis L.in Inner Mongolia

Xie Bing-ren

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Abstract

Genetic diversity of 17 Beauveria bassiana strains collected from 3 different areas of Inner Mongolia was estimated using inter-simple sequence repeat(ISSR) markers.Fifteen high reproducibility and polymorphism ISSR primers were chosen from the twenty-one samples.The optimal reactive conditions were determined for using these primers.With 15 ISSR primers,a total of 90 fragments were amplified,in which in which 87 were polymorphic.The percentage of polymorphic loci(PPL) was 96.67%.Nei's genetic diversity index(H) was 0.4309 and Shannon's genetic diversity index(I) was 0.6106.The cluster analysis of the inter-population genetic differentiation showed that the highest genetic similarity of the western part compared with the central region was 0.9897 point and the highest genetic similarity of the eastern part with the western part was 0.9621 point.

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Genetic diversity of 17 Beauveria bassiana strains collected from 3 different areas of Inner Mongolia was estimated using inter-simple sequence repeat(ISSR) markers.Fifteen high reproducibility and polymorphism ISSR primers were chosen from the twenty-one samples.The optimal reactive conditions were determined for using these primers.With 15 ISSR primers,a total of 90 fragments were amplified,in which in which 87 were polymorphic.The percentage of polymorphic loci(PPL) was 96.67%.Nei's genetic diversity index(H) was 0.4309 and Shannon's genetic diversity index(I) was 0.6106.The cluster analysis of the inter-population genetic differentiation showed that the highest genetic similarity of the western part compared with the central region was 0.9897 point and the highest genetic similarity of the eastern part with the western part was 0.9621 point.

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Available abstract

Genetic diversity of 17 Beauveria bassiana strains collected from 3 different areas of Inner Mongolia was estimated using inter-simple sequence repeat(ISSR) markers.Fifteen high reproducibility and polymorphism ISSR primers were chosen from the twenty-one samples.The optimal reactive conditions were determined for using these primers.With 15 ISSR primers,a total of 90 fragments were amplified,in which in which 87 were polymorphic.The percentage of polymorphic loci(PPL) was 96.67%.Nei's genetic diversity index(H) was 0.4309 and Shannon's genetic diversity index(I) was 0.6106.The cluster analysis of the inter-population genetic differentiation showed that the highest genetic similarity of the western part compared with the central region was 0.9897 point and the highest genetic similarity of the eastern part with the western part was 0.9621 point.

Key concepts: Genetic diversity, Biology, Genetic similarity, Veterinary medicine, Beauveria bassiana, Genetic distance, Genetic variation, Population

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Genetic Diversity of Beauveria bassiana on Loxostege sticticalis L.in Inner Mongolia — Research Paper | ScholarLens