STUDY ON THE CLEANING EFFECT OF MEDICAL INSTRUMENT MONITORED BY NINHYDRIN REAGENT

Objective To evaluate the cleaning effect of medical instrument by ninhydrin reagent.Method Monitoring the cleaning effect of medical instrument by cotton swab smeared and dipped with ninhydrin reagent,which was compared with traditional naked eye observation,vision correction method and occult blood test.Results 5 μg,0.5 μg and 0.05 μg Glycine can be detected respectively by ninhydrin reagent at 37℃、54℃ and 110℃ for 30 min.0.5 μg Glycine can be detected by ninhydrin reagent at 37℃ for 60 min.The variation of color developing reaction was significant when pH changed from 6 to 8.Ninhydrin reagent method was more sensitive for detecting residue of organism on medical instrument than OB test,vision correction method and naked eye observation.Conclusion The sensitivity of ninhydrin reagent to evaluate the cleaning effect is better than that of OB test and naked eye observation,while it is influenced by temperature,pH and color developing time.