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2015•World Academy of Science, Engineering and Technology, International Journal of Nutrition and Food EngineeringRequires access

Modified Ninhydrin Reagent for the Detection of Amino Acids on TLC Paper

Huda Shaaban Elgubbi, Adel Mohammad Mlitan, A. Alzridy

Open publisher page 2 citations

Abstract

Ninhydrin is the most well known spray reagent for identification of amino acids. Spring with Ninhydrin as a non-specific reagent is well-known and widely used for its remarkable high sensitivity. Using Ninhydrin reagent alone to detect amino acid on thin layer chromatography (TLC) paper is not advisable due to its lower sensitivity. A new spray reagent, Stannus chloride solution (SnCl 2 ) has been used to detect amino acids on filter paper (whitman paper 14) and TLC paper, silica Gel, 60 F254 TLC Aluminum Sheet 20 x 20 cm Merck- Germany. Al so, modified TLC pre-staining method was used, which only consisted of 3 steps: spotting, separating and color. The improved method was rapid and inexpensive and the results obtained were clear and reliable. In addition it is suitable for screening different amino acid.

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What this paper is about

Ninhydrin is the most well known spray reagent for identification of amino acids. Spring with Ninhydrin as a non-specific reagent is well-known and widely used for its remarkable high sensitivity. Using Ninhydrin reagent alone to detect amino acid on thin layer chromatography (TLC) paper is not advisable due to its lower sensitivity. A new spray reagent, Stannus chloride solution (SnCl 2 ) has been used to detect amino acids on filter paper (whitman paper 14) and TLC paper, silica Gel, 60 F254 TLC Aluminum Sheet 20 x 20 cm Merck- Germany. Al so, modified TLC pre-staining method was used, which only consisted of 3 steps: spotting, separating and color. The improved method was rapid and inexpensive and the results obtained were clear and reliable. In addition it is suitable for screening different amino acid.

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Available abstract

Ninhydrin is the most well known spray reagent for identification of amino acids. Spring with Ninhydrin as a non-specific reagent is well-known and widely used for its remarkable high sensitivity. Using Ninhydrin reagent alone to detect amino acid on thin layer chromatography (TLC) paper is not advisable due to its lower sensitivity. A new spray reagent, Stannus chloride solution (SnCl 2 ) has been used to detect amino acids on filter paper (whitman paper 14) and TLC paper, silica Gel, 60 F254 TLC Aluminum Sheet 20 x 20 cm Merck- Germany. Al so, modified TLC pre-staining method was used, which only consisted of 3 steps: spotting, separating and color. The improved method was rapid and inexpensive and the results obtained were clear and reliable. In addition it is suitable for screening different amino acid.

Key concepts: Ninhydrin, Reagent, Chromatography, Chemistry, Silica gel, Amino acid, Thin-layer chromatography, Filter paper

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