Secreting expression of porcine interferon-β in genetically engineered Pichia pastoris

According to Pichia pastoris preference of codon usage, the porcine interferon-β gene was synthesized and recombinant vector pPICZAPoIFN-β was constructed. The recombinant plasmid was linearized with Sac I and transformed into P. pastoris GS115 by electroporation. The engineered strain P. pastori GS115/pPICZA- PoIFN-β was identified on zeocin resistant plate. 1% methanol was used to induce the expression of GS115/pPICZA- PoIFN-β. SDS-PAGE indicated that the engineered strain efficiently secreted porcine IFN-β, with two molecular weight products of 22 ku and 26 ku, and the expression porcine IFN-β proteins were about 80 mg/L.