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Fermentation condition for higher inulinase production byAspergillus niger and selection of mutants

Jin Zheng-yu

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Abstract

The optimum fermentation condition for higher inulinase production of Aspergillus niger was determined as shown below:inulin 2%;yeast extract 2%;NH4H2PO4 05%;NaCl 05%;MgSO4·7H2O 005%;ZnSO4·7H2O 001%;pH65;inoculation 16%Maximal inulinase yield of 26U/ml was obtained after 120h of growth in shake cultures (30ml of medium in 250-ml flasks) and the value of I/S was 1.15.A mutant was isolated after treatment by Co60 .The inulinase activity was enchanced by 30% and the value of I/S was not changed.

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What this paper is about

The optimum fermentation condition for higher inulinase production of Aspergillus niger was determined as shown below:inulin 2%;yeast extract 2%;NH4H2PO4 05%;NaCl 05%;MgSO4·7H2O 005%;ZnSO4·7H2O 001%;pH65;inoculation 16%Maximal inulinase yield of 26U/ml was obtained after 120h of growth in shake cultures (30ml of medium in 250-ml flasks) and the value of I/S was 1.15.A mutant was isolated after treatment by Co60 .The inulinase activity was enchanced by 30% and the value of I/S was not changed.

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Available abstract

The optimum fermentation condition for higher inulinase production of Aspergillus niger was determined as shown below:inulin 2%;yeast extract 2%;NH4H2PO4 05%;NaCl 05%;MgSO4·7H2O 005%;ZnSO4·7H2O 001%;pH65;inoculation 16%Maximal inulinase yield of 26U/ml was obtained after 120h of growth in shake cultures (30ml of medium in 250-ml flasks) and the value of I/S was 1.15.A mutant was isolated after treatment by Co60 .The inulinase activity was enchanced by 30% and the value of I/S was not changed.

Key concepts: Inulinase, Fermentation, Food science, Inulin, Aspergillus niger, Chemistry, Yield (engineering), Inoculation

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Fermentation condition for higher inulinase production byAspergillus niger and selection of mutants — Research Paper | ScholarLens