Association of MTATP_8,MTCYB deletions with the changes of motive tracts before and after sperm capacitation

Objectives:To explore the relationship between deletions of MTATP 8,MTCYB gene and sperm capacitation defection leading to mlae infertility. Methods:The deletion of MTATP 8 and MTCYB genes before and after sperm capacitation in 60 infertile (30 with poor sperm motility and 30 with normal sperm motility) and 30 fertile males were analyzed by PCR.The changes of motive tracts of sperm were observed simultaneously. Results:Among 60 samples from infertile males,16(10 with poor sperm motility and 6 with normal motility) were found to have no change on motive tracts before and after sperm capacitation.All the 30 samples from fertile males were found to have great changes on motive tracts before and after sperm capacitation.In the 60 samples from infertile males,2 MTATP 8 deletion and 4 MTCYB deletion were detected in the 30 samples with normal motility,while 2 MTATP 8 deletion and 3 MTCYB deletion were detected in the 30 samples with poor motility.No significant difference was found between the 2 groups( P 0.05).The deletion of MTATP 8 gene or MTCYB gene was not found in the 30 samples from fertile males.The difference of MTATP 8 gene or MTCYB gene deletion between the infertile and fertile group were significant( P 0.05).In infertile males,the rate of MTATP 8 gene deletion was 7%,while the rate of MTCYB gene deletion was 12%. Conclusions:The deletion of MTATP 8 gene or MTCYB gene may affect sperm capacitation,which may lead to infertility.