Preliminary study on inhibitory effects of chitooligosaccharides phosphonate to tobacco mosaic virus(TMV)

In order to develop new agent on inhibition to tobacco mosaic virus(TMV) based on biomolecules,five new chitooligosaccharides phosphonates [2-(α)-arylamino phosphonate]-chitosan]were applied to investigate the inductive effect of inhibition to TMV infection.Results showed that all had good control on TMV after being evaluated by lesion host N.glutinosa leaves,and COS-P-3 at 200 μg·mL-1 was the most effective with inhibition rate of 90.79%,increased by 51.8% and 24.0% respectively than di(octylaminoethyl) and ningnanmycin.Chlorophyll content increased compared to inoculated Nicotiana tabacum leaves.Activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),polyphenol oxidase(PPO) and phenylalanine ammonialyase(PAL) all increased at various levels.It is concluded that tobacco treated with COS-P-3 induced the resistance to TMV by inducing defense reaction,increasing activities of preventive enzymes,and protecting synthesis of chlorophyll in leaves.