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2011•Journal of China Pediatric Blood and CancerRequires access

The study of cell damage and cell cycle arrest after high single dose irradiation in K562 cells

Zhao Qian

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Abstract

Objective To observe the type of cell damage and repair induced by 6 MV-X ray with different single dose irradiation in K562 cell lines.Methods K562 cells were exposed to 6 MV X-ray of 0～20 Gy respectively.Cell apoptosis and cell cycle progression were detected by flow cytometry(FCM) after staining with FITC-ANNEXIN V/PI or only PI at the time point of 2 h、24 h and 48 h after irradiation.The type of cell damage and the ability of cell repair caused by different dose of irradiation were analyzed.Results The percentage of cell apoptosis was increased(P0.05) with an irreversible G2/M phase cell cycle arrest after high-dose irradiation.Conclusions Lethal damage of K562 cells was observed by high-dose X-ray irradiation.The type of damage is apoptosis which could not be repaired.

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Objective To observe the type of cell damage and repair induced by 6 MV-X ray with different single dose irradiation in K562 cell lines.Methods K562 cells were exposed to 6 MV X-ray of 0～20 Gy respectively.Cell apoptosis and cell cycle progression were detected by flow cytometry(FCM) after staining with FITC-ANNEXIN V/PI or only PI at the time point of 2 h、24 h and 48 h after irradiation.The type of cell damage and the ability of cell repair caused by different dose of irradiation were analyzed.Results The percentage of cell apoptosis was increased(P0.05) with an irreversible G2/M phase cell cycle arrest after high-dose irradiation.Conclusions Lethal damage of K562 cells was observed by high-dose X-ray irradiation.The type of damage is apoptosis which could not be repaired.

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Available abstract

Objective To observe the type of cell damage and repair induced by 6 MV-X ray with different single dose irradiation in K562 cell lines.Methods K562 cells were exposed to 6 MV X-ray of 0～20 Gy respectively.Cell apoptosis and cell cycle progression were detected by flow cytometry(FCM) after staining with FITC-ANNEXIN V/PI or only PI at the time point of 2 h、24 h and 48 h after irradiation.The type of cell damage and the ability of cell repair caused by different dose of irradiation were analyzed.Results The percentage of cell apoptosis was increased(P0.05) with an irreversible G2/M phase cell cycle arrest after high-dose irradiation.Conclusions Lethal damage of K562 cells was observed by high-dose X-ray irradiation.The type of damage is apoptosis which could not be repaired.

Key concepts: Apoptosis, Flow cytometry, Irradiation, Cell cycle, Cell, Annexin, Cell damage, Cell cycle checkpoint

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