Bacteriostasis Effect Research of Cefotaxime/Sulbactam Compound Preparation to β-lactmase Production Gram Negative Bacilli in Vitro

Objective To evaluate the bacteriostasis effect of cefotaxime/sulbactam compound preparation on β-lactmase production gram negative bacilli in vitro.Methods The minimum inhibitory concentrations (MICs) of different drugs were determined by the agar dilution method;Ten kinds of ESBLs genes were identified by PCR-sequencing.Results The MIC50 of cefotaxime/sulbactam was obviously lower than cefotaxime or sulbactam,but equivalent to cefoperazone/sulbactam.Four β-lactmases genes were detected,viz tem,ctx-m,per-1 and oxa-Ⅰ.Sequencing and homology analysis confirmed they were tem-1,ctx-m-1 and oxa-10,respectively.But bacteriostasis of cefotaxime/sulbactam had no obviously difference between different β-lactmase subtypes.Conclusion cefotaxime/sulbactam had better antibacterial activity than cefotaxime alone and its bacteriostasis had no obviously difference between different β-lactmase subtypes.