Determination of the Organic and Inorganic Selenium Contents in Selenium-Enriched Yeast by Hydride Generation-Atomic Fluorescence Spectrometry

A method for the determination of organic and inorganic selenium in selenium-enriched yeast by hydride generation atomic fluorescence spectrometry(HG-AFS) has been developed.The effects of the instrumental and chemical parameters on atomic fluorescence signal of selenium were investigated,and the optimum conditions were recommended.The effects of digest reagents on digestion of samples and the interference from foreign ions on selenium were briefly discussed.The detection limit for selenium is 0.0785 μg/L under the optimum condition.The linear range is 0～100 μg/L.The relative standard derivation is less than 0.45%.A total selenium content of 1.40～3.20 mg/g and the organic selenium content of 1.30～2.97 mg/g were detected respectively from the selenium enriched yeast by this method,and the recoveries were 95.4%～104.3%.