The role of arsenic trioxide on lymphangiogenesis of transplantation tumor model for humen breast infitrating ductal carcinoma in nude mice

Objective:To investigate the effect of arsenic trioxide on antitumor lymphangiogenesis for breast cancer.Methods:Twenty four nude mices bearing transplantation tumor were randomly divived into four groups:negative control group(saline),positive control group(5-Fluorouracil 30mg/kg),experimental group 1(As2O3 1.5mg/kg) and experimental group 2(As2O3 3.0mg/kg).Immunohistochemical staining and reverse transcription-polymerse chain reaction were used to detect the expression of COX-2 and VEGF-C proteins and mRNA.Results: The positive rates of expression of COX-2、VEGF-C protein and mRNA were high in negative group.The expression of COX-2、VEGF-C in experimental 1 and 2 was decreased with the increase of dosage of As2O3(P0.05),and showed a remarkable descending tendency compared with negative and positive control groups(P0.05).The expression of COX-2、VEGF-C showed positive correlation in each groups(r=0.725,r=0.915) .Conclusions:As2O3 has antitumor lymphangioigenesis activity by decreasing the expression of COX-2 and VEGF-C.