Regulation of Glucocorticoids and Radix Astragali Comparison study of TGF-β1/Smad3 Signal Pathway on Airway Remodelling in Asthma Rats

Objective:The regulation of Glucocorticoids and Radix Astragali(RA) toTGF-β1/ Smad3 signal pathway and asthma airway remodeling was observed.Methods:In the experiment,50 male Sprague-Dawlay(SD) rats,grade SPF were needed.Asthmatic airway remodelling model rats were sensitized with ovalbumin and AL(OH)3,and repeatedly exposed to aerosolized ovalbumin.According to the repeated time course of challenge,rats were randomly divided into the control group(group A),asthma group(group B) and treated group with BUD(group C),treaded group with DXM(group D),treated group with RA(group F).Every group has ten rats.The total brochial wall thickness(Wat) and the airway smooth muscle thickness(Wam) were measured by image analysis system.The protein expressions of TGF-β1 and P-Smad3 in the lung tissues were detected by immunohistochemistry technique.The concentrations of TGF-β1 in serum and BALF were tested by sandwich ELISA.Results:Compared with group B,Wat and Wam of treated groups were significantly decreased(P0.01),group C and D were lower than group F.The protein expression of TGF-β1 was obviously consistent with that of P-Smad3.Protein expressions of TGF-β1 and P-Smad3 in treated groups were significantly elevated compared with group B,group C and D were significantly lower than group F(P0.01).The concentrations of TGF-β1 in serum and BALF:treated groups were significantly decreased than group B,in BALF,group C nad group D were the same with group F,but in serum,group C and group D were lower than group F.Conclusions:BUD,DXM and Radix Astragali(RA) markedly reduce the airway inflammation,inhibit and improve the airway remodeling,which is possibly partly through regulating TGF-β1/ Smad3 pathway,but Glucocorticoids were better effective than RA.