2012•Strait Pharmaceutical JournalRequires access
Effect of early intervention of budesonide on expressions of TGF-β_1/Smad in rats with acute asthma
Tong Xia-sheng
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Abstract
OBJECTIVE To discuss the effect of early intervention of budesonide on expressions of TGF-β1/Smad in rats with acute asthma.METHODS Thirty Wistar rats were randomly divided into 3 groups:control group(group C),asthma model group(group A)and budesonide therapy group(group B),with 10 rats in each group.The model of asthma was established by the ovalbumin(OVA) challenge methods and the group B was received early therapy by inhaling budesonide.The concentration of TGF-β1 in BALF and serum were detected by ELISA.The expression of TGF-β1,phosphorylated Smad2/3(P-Smad2/3) and Smad7 proteins in lung tissue were detected by immunocytochemical method.RESULTS In BALF or serum,the concentration of TGF-β1 in group C was lower than that in group A or group B respectively.The concentrations of TGF-β1 in BALF or serum in group B was lower than that in group A.The expressions of P-Smad2/3 of group A in lung tissue was higher than that in the other two group respectively.The expressions of Smad7 of group A in lung tissue was the lowest in the three group.CONCLUSION The early intervention of budesonide could decrease the expression of TGF-β1 and P-Smad2/3,while increase the expression of Smad7 in rats with acute asthma.