Genetic effects analysis of male sterile cytoplasm and nucleo-cytoplasmic interactions in CMS-FA hybrid rice

Using 4 CMS-FA sterile lines(A line) and the corresponding maintainer line(B line) as female parents,and 3 corresponding restorer lines(R line) as male parents,12 gemination hybrids of A lines×R lines(AF1) and B line×R lines(BF1) were made to analyze the genetic effects of male sterile cytoplasms and nucleo-cytoplasmic on yield-related traits of CMS-FA hybrid rice by using the difference(AF1-BF1)/BF1×100% and additive-dominant×cytoplasmic modle.The results showed significant positive effects on 1000-grain-weight(TGW) and growth duration(GD) and significant negative effects on panicle neck length(PNL) were observed.The cytoplasmic effects on other eight traits were insignificant.Significant interaction effects of nucleus and cytoplasm were observed on panicle number per plant,TGW,total grain number per plant,seed-set percent,plant height(PH),panicle length(PL),PNL,and GD.Because of the interaction effects,the prediction of F1 genotype value,their population mean heterosis and population better-parent heterosis were increased in PH and PNL.Male sterile cytoplasm of CMS-FA hybrid rice was no adverse cytoplasmic effect,its sterile lines were well resumed,as an excellent cytoplasmic it can be used for breeding of hybrid rice.