Detection of the class 1 integrase gene and plasmid-AmpC enzyme genes in Enterobacter cloacae

Objective To investigate the genes of the class 1 integrase(intⅠ1), qacE△1-sulⅠ, and plasmid-AmpC enzyme(AmpC MIR and AmpC DHA) in Enterobacter cloacae. Methods 20 strains of Enterobacter cloacae were isolated from the sections of in-patients, and their susceptibilities of 20 kinds of antimicrobial agents against these strains by microdilute tests. Genotypes was analyzed by polymerase chain reaction (PCR). Results 20 strains of Enterobacter cloacae showed multiple-drug resistance. There is no strain resistant to Imipenam and Meropenem. The resistant rates to Cefepime and Cotrimoxazol were 25.0% and 85.0% respectively, and with 60.0% to 100.0% of other antimicrobial agents. The positive rates of intⅠ1, qacE△1-sulⅠ, MIR, and DHA genes were 95.0%,85.0%,85.0%, and 5.0% respectively. Conclusions There are very high positive rate of intⅠ1,qacE△1-sulⅠ, and plasmid-AmpC MIR genes in Enterobacter cloacae isolated from the sections of in-patients in our hospital. The genes of intⅠ1, qacE△1-sulⅠ, and plasmid-AmpC MIR in Enterobacter cloacae are firstly reported in P.R.C.