Study on Extraction of Berberine from Coptis chinensis by Polysorbate-80

Objective To study the including effect of polysorbate-80 on extraction of berberine from Coptis chinensis.Methods Orthogonal experiment design was conducted to optimize excraction conditions.Three factors,including the content of polysorbate-80,concentration of sulfuric acid,content of sulfuric acid,were considered.Results The optimum conditions of extraction were obtained as follows: ratio of solid-liquid of polysorbate-80 was 2∶3,concentration of sulfuric acid was 0.2 %,ratio of solid-liquid of sulfuric acid was1∶35.Conclusion Polysorbate-80 can promote the extracting rate of berberine from coptis chinensis.