The changes of Th1/Th2 under Salmonella typhimurium infection in mice

Objective To study the changes of Th1/Th2 under Salmonella typhimurium infection in mice Methods 24 BALB/c mice were randomly divided into 2 groups,including infection group and control group.The mice of infection group were orally infected with STM by a single dose,and were sacrificed on series time points.Spleen,liver and small intestine was observed with microscope.Interferon-γ(IFN-γ)and interleukin-4(IL-4) of splenocytes was detected with flow cytometry,which represented the numbers of Th1 and Th2,respectively.Results Spleen,liver and small intestine was characterized by infiltrated with neutrophil leukocyte,monocyte and lmyphocyte.After 24 hours infection,the concentration of IFN-γ in spleen began to increase,reached the peak at 3 day after infection.The increased IFN-γ lasted for 5 days.The results indicated that the number of Th1 was increased under infection.Conclusions After STM infection,cell immune response switched to Th1 type immune response.The exacerbated inflammation in small intestine of host resulted from Th1 cell immune increased in the early stage.