Experimental study on Effect of Feixingqing Capsules in Treating Pulmonary Fibrosis in Rat and it's cytokine TGF-β1、Smad2/3

Objective To investigate the effects of Feixingqing Capsules in boleomycin-induced pulmonary fibrosis in rat and the expression of TGF-β1、Smad2/3 in the lung,and to explore the possible mechanism in pulmonary fibrosis.Methods Thirty-two healthy male SD rats were randomly divided into 4 groups(n=8),the normal control group(CG),the model group(MG),the positive control prednisone group(DG) and FXQ group(EG).Except those rats in normal control group,all rats were established pulmonary fibrosis by intra tracheal administration of bleomyci,CG were injected with equivalent dosage of saline.After the model of fibrosis was established for 24 h,the rats of DG,EG were given the drug mentioned above respectively,while only saline was given in CG and MG.On day 28 day after treatment,each group were sacrificed respectively,and the lungs were harvest for conventional HE pathological obserbation.TGF-β1 and Smad2/3 protein expression in lung issues were detected by immunohistochemistry.Results Compared with the MG,PXQ can effectively reduce the degree of inflammation and fibrosis,and the expression of TGF-β1 and Smad2/3 in the lung were decreased.Conclusions Feixingqing Capsules have a treatment effect on pulmonary fibrosis,possible mechanism is through inhibiting the expression of TGF-β1 and Smad2/3 in the lung of rats with pulmonary.