Study on Biology Characteristic of Botrytis cinerea Pers
Xiying Li
Abstract
Xiying Li
Abstract
[Objective]The study aimed to systematically research the biology characteristic of Botrytis cinerea Pers.[Method]The diseased tissues with typical symptom from B.cinerea were selected in the field.The pathogenic bacteria was isolated by tissue segregation method and got pure culture so as to study the effects of medium,temperature,pH value,carbon source,nitrogen source on the growth characteristic of B.cinerea spores.[Result]The optimum temperature for the hypha growth and sporulation of B.cinerea was 25 ℃.The temperature range of conidia germination was 20-25 ℃.And the optimum pH value for the germ hypha growth and conidia germination was 6.0.The optimum carbon source was sucrose and the second was glucose and fructose.The optimum nitrogen source was peptone and the second were beef extract,yeast extract,alanine and ammonium nitrate.Among the media,the growth of hypha cultured from PDA medium was fastest,with the production of gray hypha and dense colonies.The lethal temperature of the sclerotia,hypha and conidia germination was 60,55 and 50 ℃.[Conclusion]The study provided the scientific basis for the research on the disease rule of B.cinerea and its prevent and control.
A significance statement is not available in the OpenAlex record.
A contribution statement is not available in the OpenAlex record.
Method details are not available in the OpenAlex metadata.
Findings are not separately available in the OpenAlex metadata.
Limitations are not available in the OpenAlex metadata.
Application details are not available in the OpenAlex metadata.
[Objective]The study aimed to systematically research the biology characteristic of Botrytis cinerea Pers.[Method]The diseased tissues with typical symptom from B.cinerea were selected in the field.The pathogenic bacteria was isolated by tissue segregation method and got pure culture so as to study the effects of medium,temperature,pH value,carbon source,nitrogen source on the growth characteristic of B.cinerea spores.[Result]The optimum temperature for the hypha growth and sporulation of B.cinerea was 25 ℃.The temperature range of conidia germination was 20-25 ℃.And the optimum pH value for the germ hypha growth and conidia germination was 6.0.The optimum carbon source was sucrose and the second was glucose and fructose.The optimum nitrogen source was peptone and the second were beef extract,yeast extract,alanine and ammonium nitrate.Among the media,the growth of hypha cultured from PDA medium was fastest,with the production of gray hypha and dense colonies.The lethal temperature of the sclerotia,hypha and conidia germination was 60,55 and 50 ℃.[Conclusion]The study provided the scientific basis for the research on the disease rule of B.cinerea and its prevent and control.
Key concepts: Botrytis cinerea, Hypha, Conidium, Germ tube, Germination, Spore, Biology, Sucrose