2004•Xibei zhiwu xuebaoRequires access
Characterization of callus induction and plant regeneration in immature embryo culture among several heterotic groups of elite maize inbreds
Zhang Hongmei, Xiaohong Liu, Zhang Hong-wei, XJ Liu, Gang Chen, Zhen-Bo Tan
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Abstract
19 maize inbreds belonging to 3 maize heterotic gruops including Reid, Tangsipingtou and compound germpasm were studied to determine the influence of genotypes, media and plant regulators on the embryo-derived callus induction and green plant regeneration. Immature embryos (1.0-2.0 mm) from selfed ears were harvested as explants. Callus could be induced from all the tested inbreds. However, plant regeneration was only obtained in 12 genotypes. N6 and modified N6 medium were proved better than other media tested for callus quality and growth. 2, 4-D played an important role in callus induction. 0.2 mg/L 6-BA or KT added in induction media lowered callus quality and induction rate of embryonic callus. However, 1 mg/L KT added in the medium of embryoid induction increased plant regeneration and also increased regeneration of multiple plantlets from a single embryo. Among 19 maize genotypes studied, 4 elite inbreds belonging to group Tangsipingtou showed poor regeneration of plants. However, elite inbred 3189/4380, 4380/Shanzong 5, 8103, Xianzao 17, 18-599 hong, 18-599 bai, 501. 178 and Ji 53 belonging to group Reid or compound germplasm were identified with higher performance both in induction of embryonic callus and plant regeneration.