Optimization of Water Extraction and Alcohol Precipitation Technology for Jianwei Qingchang Mixtures by Orthogonal Test

Objective:To optimize water extraction and alcohol precipitation technology of Jianwei Qingchang mixtures.Method:With composite score of the total content of rhein,emodin and rhubarb phenol,dry extract yield as index,effects of the amount of water,extraction times and time on extraction technology was investigated by orthogonal test;With the total content of rhein,emodin and rhubarb phenol as index,orthogonal test was adopted to investigate effects of the concentration of alcohol precipitation and relative density of concentrate on alcohol precipitation technology.Result:Optimum water extraction and alcohol precipitation technology was as following:extracted 2 times with 10 folds the amount of water,3 h each time,extracts were combined and concentrated to a relative density of 1.05 g.mL-1,added 95% ethanol to precipitate the volume fraction of ethanol with 60%.Conclusion:Optimized water extraction and alcohol precipitation technology was stable and feasible,it could provide a reference for standardized production of Jianwei Qingchang mixtures.