Genetic diversity study of Forsythia suspensa populations by ISSR in Henan

By using inter simple sequence repeat(ISSR) marker technique,the genetic diversity of six natural populations of Forsythia suspensa growing in different regions in Henan province were investigated.The results show that the Forsythia suspense species have higher genetic diversity.89 bands were amplified from 6 natural populations by 12 reliable primers.The amplification results were analyzed by the software POPGENE 32.At the species level,the percentage of polymorphic loci was 94.38%;Nei’s gene diversity index(H) was 0.3265,Shannon information index(I) was 0.4901;the genetic differentiation coefficient was 0.2743 which indicated 72.57% of genetic variation existed within populations.A dendrogram showed that the genetic relationship was constructed through an unweighted pair group method(UPGMA) based on the genetic identity and 33 F.suspensa materials were clustered into 2 main groups and 6 subgroups.It was found that the genetic distance and geographic distance were un-correlated,the genetic variation pattern of F.suspense in Henan might be ascribed to heterogeneous habitats and biological characteristic of this species.