ISSR analysis on genetic diversity of Ambrosia artemisiifolia L. from different population in Hunan province

Genetic diversity and genetic differentiation of A.artemisiifolia were studied by using ISSR makers,ISSR amplification was conducted with 6 primers for 75 individuals from 5 populations.The results showed that 29 loci amplified polymorphic were detected,of which 25(PPL=86.21%) were polymorphic loci.Genetic diversity analysis showed that Nei's gene diversity(He) was 0.2545 and Shannon's genetic diversity index(I) was 0.3125 in species,it indicated high genetic variation at species level.The total genetic diversity(Gst) was 0.2961.Genetic differentiation mainly occurred within populations(70.39%),and without populations(29.61%).UPGMA cluster analysis indicated that two populations of Changsha and Linxiang clustered in a clade,Jiangyong and Lingling clustered in an other clade,the two clade clustered in aclade at last.Genetic distance was correlated with geographical distance.