Influence of co-transfusion of mesenchymal stem cells and bone marrow cells before islet transplantation on cytokines of diabetic rat

Objective To investigate the influence of co-transfusion of mesenchymal stem cells and bone marrow cells before islet transplantation on cytokines in diabetic rat.Methods Animal model of diabetes mellitus was established.MSCs from Wistar rats were purified through passage culture.The donor mesenchymal stem cells and/or bone marrow cells were injected into the recipient SD rats via veins,and diabetic SD rats received islet transplantation 7 days after the injection.Survival time of the islets grafts was observed,and the level of serum IL-2,IFN-γ,IL-4,IL-10 and TGF-β1 were detected.Results The pre-infusion of MSCs and BMCs significantly prolonged allograft survival time(MST 30 d).The contents of IL-2 and INF-γ were reduced while the contents of IL-10 and TGF-β1 were increased significantly after co-infusion of high concentration of MSCs and BMCs.Conclusion The contents of cytokines can be changed by co-transfusion of high-concentration MSCs and BMCs,which might be one of the reasons to induce pancreatic islets transplantation tolerance.