Combination Transplantation of Rat Islet Cells and Bone Marrow Mesenchymal St em Cells for Diabet ic Mouse

Objective To observe the immune tolerance of bone marrow mesenchymal stem cells induced xenogenic islet cell transplantation.Methods We used 4-week-old SD rats as donors,the centrifugation of mesenchymal stem cells was performed by using density gradient,the separation of islet cells by collagenase digestion,and the preparation of of 25 STZ-diabetic model BALB/C mice with Streptozotocin.The model mice were divided into three groups:group A(combination transplantation of rat islet cells and bone marrow mesenchymal stem cells in10 mice),group B(islet cell transplantation by the portal vein injection into the liver channel in 10 mice)and Group C(control group,normal saline by portal vein injection in 5 mice.The blood glucose improvement in each group was compared.Results Groups A and B showed a significant difference compared with Group C,with the effect of Group A superior to that of Group B.Conclusion Bone marrow mesenchymal stem cells can induce the immune tolerance of the mice to the rat islet cells and extend the survival time of islet cell graft.