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2008•Journal of Southwest UniversityRequires access

An Effective Way for the Construction of Recombinant Adenoviral Vector Encoding the Antigen of Mycobacterium tuberculosis

Chen Shui

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Abstract

On the basis of AdEasy Adenoviral System,a simple and effective method for constructing the recombinant adenoviral vector encoding the antigen of M.tuberculosis was established.Up to 90% of the picked clones were positive.The green fluorescence from GFP(green fluorescence protein) reporter of 293A cells transfected with the linearized recombinant adenoviral plasmid was observed after 24 h.

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What this paper is about

On the basis of AdEasy Adenoviral System,a simple and effective method for constructing the recombinant adenoviral vector encoding the antigen of M.tuberculosis was established.Up to 90% of the picked clones were positive.The green fluorescence from GFP(green fluorescence protein) reporter of 293A cells transfected with the linearized recombinant adenoviral plasmid was observed after 24 h.

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Available abstract

On the basis of AdEasy Adenoviral System,a simple and effective method for constructing the recombinant adenoviral vector encoding the antigen of M.tuberculosis was established.Up to 90% of the picked clones were positive.The green fluorescence from GFP(green fluorescence protein) reporter of 293A cells transfected with the linearized recombinant adenoviral plasmid was observed after 24 h.

Key concepts: Recombinant DNA, Virology, Green fluorescent protein, Mycobacterium tuberculosis, Vector (molecular biology), Viral vector, Plasmid, Transfection

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An Effective Way for the Construction of Recombinant Adenoviral Vector Encoding the Antigen of Mycobacterium tuberculosis — Research Paper | ScholarLens