ScholarLens Back to search
2000•Academic Journal of Sun Yat-sen University of Medical SciencesRequires access

Construction of Recombinant Adenoviral Vector expressing HBsAg

Zhi Zhou, Yao Ji-lu, Lin Yang, Deng Lian-xian, Xuejuan Chen

Open publisher page 0 citations

Abstract

Objective To explore the use of adenoviral vector in gene therapy and the effectiveness of target antigen expression in it. Method S gene was released from pBluscript KS +-HBs by BamHⅠ、EcoRⅠ cutting, then inserted directionally into plasmid pACCMVpL. The recombinant vector was identified by restriction endonuclease analysis. Result All the 8 clones selected were recombinant vector, which release about 950 bp target gene fragment by BamHⅠ、EcoRⅠ digestion. Conclusion The adenoviral vector which expressed HBsAg was constructed successfully.

About this research paper

What this paper is about

Objective To explore the use of adenoviral vector in gene therapy and the effectiveness of target antigen expression in it. Method S gene was released from pBluscript KS +-HBs by BamHⅠ、EcoRⅠ cutting, then inserted directionally into plasmid pACCMVpL. The recombinant vector was identified by restriction endonuclease analysis. Result All the 8 clones selected were recombinant vector, which release about 950 bp target gene fragment by BamHⅠ、EcoRⅠ digestion. Conclusion The adenoviral vector which expressed HBsAg was constructed successfully.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To explore the use of adenoviral vector in gene therapy and the effectiveness of target antigen expression in it. Method S gene was released from pBluscript KS +-HBs by BamHⅠ、EcoRⅠ cutting, then inserted directionally into plasmid pACCMVpL. The recombinant vector was identified by restriction endonuclease analysis. Result All the 8 clones selected were recombinant vector, which release about 950 bp target gene fragment by BamHⅠ、EcoRⅠ digestion. Conclusion The adenoviral vector which expressed HBsAg was constructed successfully.

Key concepts: Recombinant DNA, HBsAg, Vector (molecular biology), Restriction enzyme, Viral vector, Plasmid, Molecular biology, Virology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Construction of Recombinant Adenoviral Vector expressing HBsAg — Research Paper | ScholarLens