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2014•Shiyong yaowu yu linchuangRequires access

Determination of ketoprofen in ketoprofen gel by RP-HPLC

Lei Shi

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Abstract

Objective To establish a RP-HPLC method for the determination of ketoprofen in ketoprofen gel.Methods The separation was performed with a Diamonsil C18column(4. 6 mm × 200 mm,5 μm) at room temperature,with the mobile phase of 0. 01 mol /L potassium phosphate(adjust with phosphoric acid to pH value of 3. 0)-acetonitrile(50∶ 50). The flow rate was 1 mL /min. Ketoprofen was detected at 255 nm. Results There was a good linear relationship between A and concentration over the range of 0. 525 ~ 1. 575 μg(r = 0. 999 9,n = 5). The average recovery was 98. 41%,RSD was 1. 35%. After light irradiation,the content of ketoprofen decreased obviously and the impurity A ketoprofen increased significantly. Conclusion This method is applicable for the determination of ketoprofen in ketoprofen gel.

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What this paper is about

Objective To establish a RP-HPLC method for the determination of ketoprofen in ketoprofen gel.Methods The separation was performed with a Diamonsil C18column(4. 6 mm × 200 mm,5 μm) at room temperature,with the mobile phase of 0. 01 mol /L potassium phosphate(adjust with phosphoric acid to pH value of 3. 0)-acetonitrile(50∶ 50). The flow rate was 1 mL /min. Ketoprofen was detected at 255 nm. Results There was a good linear relationship between A and concentration over the range of 0. 525 ~ 1. 575 μg(r = 0. 999 9,n = 5). The average recovery was 98. 41%,RSD was 1. 35%. After light irradiation,the content of ketoprofen decreased obviously and the impurity A ketoprofen increased significantly. Conclusion This method is applicable for the determination of ketoprofen in ketoprofen gel.

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Available abstract

Objective To establish a RP-HPLC method for the determination of ketoprofen in ketoprofen gel.Methods The separation was performed with a Diamonsil C18column(4. 6 mm × 200 mm,5 μm) at room temperature,with the mobile phase of 0. 01 mol /L potassium phosphate(adjust with phosphoric acid to pH value of 3. 0)-acetonitrile(50∶ 50). The flow rate was 1 mL /min. Ketoprofen was detected at 255 nm. Results There was a good linear relationship between A and concentration over the range of 0. 525 ~ 1. 575 μg(r = 0. 999 9,n = 5). The average recovery was 98. 41%,RSD was 1. 35%. After light irradiation,the content of ketoprofen decreased obviously and the impurity A ketoprofen increased significantly. Conclusion This method is applicable for the determination of ketoprofen in ketoprofen gel.

Key concepts: Ketoprofen, Chromatography, Chemistry, High-performance liquid chromatography, Phosphoric acid, Potassium, Organic chemistry

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