A study on cells proliferation and invasiveness of Hep-2 inhibited by RNA interference mediated PIK3CA gene silencing

OBJECTIVE To observe the effects of RNA interference mediated PIK3CA gene silencing on the proliferation and invasiveness of laryngeal squamous cell carcinoma(LSCC) cells, and investigate the feasibility of PIK3CA gene as a potential therapeutic target in the treatment of LSCC. METHODS The lentiviral vector system expressing short hairpin RNA targeting PIK3CA gene(PIK3C-shRNA)was constructed and transfected subsequently into Hep-2 cells mediated by liposome in vitro. The expression of PIK3CA gene was detected by real-time RT-PCR and Western blot respectively. The proliferation of Hep-2 cells was measured by MTT, colony formation, and cell growth curve. The invasive power was determined by Boyden chamber model in vitro. RESULTS The lentiviral vector system expressing short hairpin PIK3CA- shRNA was constructed successfully. Compared with the control groups, the mRNA and protein expression of PIK3CA were significantly down-regulated(75% and 70% respectively)in the experimental group (P0.01). The cell proliferation and invasive power were significantly inhibited in vitro(P0.05). CONCLUSION The results suggest that shRNA mediated PIK3CA gene silencing can inhibit the proliferation and invasiveness of Hep-2 cells in vitro. PIK3CA has the possibility to be a new candidate gene for gene therapy of LSCC.