Effect of miRNA-224-5p gene silencing on cell proliferation,apoptosis and invasion in human laryngeal cancer cell line Hep-2 by RNA interference

OBJECTIVE To explore the influence of miRNA-224-5p gene silencing on cell proliferation,apoptosis and invasion in human laryngeal cancer cell line Hep-2 by lentiviral vector-mediated RNA interference.METHODS miRNA-224-5p specific siRNA gene was synthesized and cloned into the recombinant lentiviral vector.Hep-2 cells were infected by miRNA-224-5p-siRNA recombinant lentivirus(miRNA-224-5p-siRNA-LV3).The Hep-2 cells were divided into SI group,NC group and BC group in vitro.The expression of the targets of miRNA-224-5p was detected by RT-PCR.Cell proliferation was detected by CCK8 kit.Cell cycle and apoptosis were detected by flow cytometry.The invasion was detected by Transwell test.RESULTS The expression level of miRNA-224-5p was inhibited significantly by miRNA224-5p-siRNA-LV3.The proliferation of Hep-2 was also markedly suppressed.The number of invasive cells that migrated through the chamber decreased.The cell cycle and apoptosis had no obvious effect.CONCLUSION MiRNA-224-5p gene silencing by RNAi can suppress the proliferation and reduce the invasiveness of Hep-2 cells.