Effect of terbutaline on absorption of lung edema in rabbits with pulmonary edema after seawater drowning

Objective To observe the effect of terbutaline on absorption of lung edema in rabbits with pulmonary edema after seawater drowning.Methods 36 ventilated New Zealand rabbits were instilled by seawater (4 mL/kg body weight) into lower tracheal and then were randomly divided into the normal group(NG),the control group(CG) and the terbutaline therapy group(TG).PaO2,PaCO2 were detected during the experiment.After treatment of 180 minutes the rabbits were killed to get lung tissues for examination of pathologic.Extravascular lung water index (EVLWI),Evan's blue leakage index (ELI) and the Na+-K+-ATPase activity of lung homogenate were analyzed.Lung tissue epithelial Na+ channel subunit-α(α-ENaC)mRNA,Na+/K+-adenosine triphosphatase subunit-α1(NKA-α1) protein abundances were measured by reverse transcriptase-polymerase chain reaction and Western blotting.Results The TG's PaO2,Na+-K+-ATPase,NKA-α1 protein and activity α-ENaC mRNA abundances were significantly higher than the CG's (P0.05),but the TG's PaCO2,EVLWI and ELI was markedly lower than the CG's(P0.05).Conclusion Terbutaline can improve hypoxia of PE-SDW rabbits.With up-regulation of the NKA activity and expressions of α-ENaC mRNA and NKA-α1,terbutaline can promote the absorption of lung edema in rabbits with seawater instillation-induced pulmonary edema.